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melanocyte cell line  (ATCC)


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    Structured Review

    ATCC melanocyte cell line
    Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and <t>melanocytes</t> (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.
    Melanocyte Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7979 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Mitochondrial fatty acid oxidation is stimulated by red light irradiation"

    Article Title: Mitochondrial fatty acid oxidation is stimulated by red light irradiation

    Journal: Febs Letters

    doi: 10.1002/1873-3468.70195

    Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and melanocytes (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.
    Figure Legend Snippet: Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and melanocytes (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.

    Techniques Used: Irradiation

    Related Articles

    Tumor Implantation:

    Article Title: Intratumoral Treatment of Melanoma Tumors with Large Surface Area Microparticle Paclitaxel and Synergy with Immune Checkpoint Inhibition
    Article Snippet: .. Tumor Implantation and Treatment The original melanocyte cell line Clone M-3 [Cloudman S91 melanoma] was acquired from the American Type Cell Collection (ATCC Cat. No. CCL-53.1) and passaged once in vivo, resulting in clone M3(Z1) used in the study. .. Cells were grown at 37.5°C, 5% CO2 in RPMI 1640 Glutamax 1 (Gibco by Life Technologies, Paisley, UK), 10% FCS, 1% penicillin/streptomycin and expanded as 70–90% confluent cultures routinely split using trypsin/EDTA.

    In Vivo:

    Article Title: Intratumoral Treatment of Melanoma Tumors with Large Surface Area Microparticle Paclitaxel and Synergy with Immune Checkpoint Inhibition
    Article Snippet: .. Tumor Implantation and Treatment The original melanocyte cell line Clone M-3 [Cloudman S91 melanoma] was acquired from the American Type Cell Collection (ATCC Cat. No. CCL-53.1) and passaged once in vivo, resulting in clone M3(Z1) used in the study. .. Cells were grown at 37.5°C, 5% CO2 in RPMI 1640 Glutamax 1 (Gibco by Life Technologies, Paisley, UK), 10% FCS, 1% penicillin/streptomycin and expanded as 70–90% confluent cultures routinely split using trypsin/EDTA.

    Article Title: Intratumoral Treatment of Melanoma Tumors with Large Surface Area Microparticle Paclitaxel and Synergy with Immune Checkpoint Inhibition
    Article Snippet: .. The original melanocyte cell line Clone M-3 [Cloudman S91 melanoma] was acquired from the American Type Cell Collection (ATCC Cat. No. CCL-53.1) and passaged once in vivo, resulting in clone M3(Z1) used in the study. .. Cells were grown at 37.5°C, 5% CO 2 in RPMI 1640 Glutamax 1 (Gibco by Life Technologies, Paisley, UK), 10% FCS, 1% penicillin/streptomycin and expanded as 70–90% confluent cultures routinely split using trypsin/EDTA.

    Cell Culture:

    Article Title: Mitochondrial fatty acid oxidation is stimulated by red light irradiation
    Article Snippet: .. A human immortalized keratinocyte cell line (HaCaT, RRID:CVCL_0038 [ ]), an immortalized skin fibroblast cell line (Hs68, RRID:CVCL_0839), and a melanocyte cell line (B16F10, RRID:CVCL_0159) were acquired from ATCC, validated within the last 3 years, confirmed mycoplasma‐free every 6 months, and cultured in high‐glucose Dulbecco modified Eagle medium (DMEM) with phenol red (Gibco, Life Technologies, Waltham, MA, USA), supplemented with 10% v/v fetal bovine serum (FBS; Sigma, St. Louis, MI, USA), 110 mg·mL −1 sodium pyruvate, 4 m m l ‐glutamine, 100 U·mL −1 of penicillin, and 100 pg·mL −1 streptomycin (Gibco, Life Technologies) at pH 7.4, 37 °C in a humidified atmosphere of 5% CO 2 . ..

    Modification:

    Article Title: Mitochondrial fatty acid oxidation is stimulated by red light irradiation
    Article Snippet: .. A human immortalized keratinocyte cell line (HaCaT, RRID:CVCL_0038 [ ]), an immortalized skin fibroblast cell line (Hs68, RRID:CVCL_0839), and a melanocyte cell line (B16F10, RRID:CVCL_0159) were acquired from ATCC, validated within the last 3 years, confirmed mycoplasma‐free every 6 months, and cultured in high‐glucose Dulbecco modified Eagle medium (DMEM) with phenol red (Gibco, Life Technologies, Waltham, MA, USA), supplemented with 10% v/v fetal bovine serum (FBS; Sigma, St. Louis, MI, USA), 110 mg·mL −1 sodium pyruvate, 4 m m l ‐glutamine, 100 U·mL −1 of penicillin, and 100 pg·mL −1 streptomycin (Gibco, Life Technologies) at pH 7.4, 37 °C in a humidified atmosphere of 5% CO 2 . ..



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    Image Search Results


    Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and melanocytes (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.

    Journal: Febs Letters

    Article Title: Mitochondrial fatty acid oxidation is stimulated by red light irradiation

    doi: 10.1002/1873-3468.70195

    Figure Lengend Snippet: Red light stimulation of OCRs is cell‐specific. Fibroblasts (A–D) and melanocytes (E–H) were treated under similar conditions to Fig. after 2 h of irradiation at 6, 12, 36, and 150 J·cm −2 with red light (660 nm), and oxygen consumption rates (OCRs) were quantified. Basal (Panel B, F), ATP production‐linked (Panel C, G), and maximal OCRs (Panel D, H) were calculated as described in Methods. Results are expressed as means ± SD of three independent experiments; ns, nonsignificant; * P < 0.05, one‐way ANOVA followed by Dunnett's test.

    Article Snippet: A human immortalized keratinocyte cell line (HaCaT, RRID:CVCL_0038 [ ]), an immortalized skin fibroblast cell line (Hs68, RRID:CVCL_0839), and a melanocyte cell line (B16F10, RRID:CVCL_0159) were acquired from ATCC, validated within the last 3 years, confirmed mycoplasma‐free every 6 months, and cultured in high‐glucose Dulbecco modified Eagle medium (DMEM) with phenol red (Gibco, Life Technologies, Waltham, MA, USA), supplemented with 10% v/v fetal bovine serum (FBS; Sigma, St. Louis, MI, USA), 110 mg·mL −1 sodium pyruvate, 4 m m l ‐glutamine, 100 U·mL −1 of penicillin, and 100 pg·mL −1 streptomycin (Gibco, Life Technologies) at pH 7.4, 37 °C in a humidified atmosphere of 5% CO 2 .

    Techniques: Irradiation